The Antioxidant and Antibacterial Properties of Fresh Mediterranean Sea Cucumber

Abstract

Mediterranean sea cucumber is one of the reach sources of undetected activities and active ingredients. The current study was conducted to find out the antioxidant and antibacterial properties of Mediterranean fresh sea cucumber, the selected pathogenic bacteria were as Staphylococcus aureus and Pseudomonas aeruginosa. The DPPH assay was used to detect the ability of fresh sea cucumber extract to inhibit free radical cells activity, while microtiter plates assay was also used to evaluate the ability of fresh sea cucumber extracts to reduce or inhibit the growth of selected Pathogenic Bacteria. Over all findings, S. aureus and P. aeruginosa were sensitive to all fresh Mediterranean sea cucumber extracts, the highest Inhibition percentage of Sterile distilled water extract was as 100% against both of S. aureus and P. aeruginosa while the strongest inhibition percentage of Ethanol extract was as 82.38% against S. aureus and as 96.42% against P. aeruginosa, the maximum inhibition percentage of Methanol extract was as 97.50% against S. aureus and as 100% against P. aeruginosa, which means that there was a clear noticeable antibacterial activity for each extract regardless to the used solvent and utilized concentrations and there wasn’t any bacterial resistance. Thus, the results showed that the highest antioxidant efficacy was back to aqueous fresh sea cucumber extract, which was as 59.22% at 5000 ppm and the IC₅₀ value was 116.27, while the strongest antioxidant activity of Ethanol fresh sea cucumber extract was as 48.94% at 2000 ppm and the IC₅₀ value was at 63.37, ultimately the maximum antioxidant ability of Methanol fresh sea cucumber extract was as 52.29% at 3000 ppm and the IC₅₀ value was at 53.38. In conclusion, fresh Mediterranean sea cucumber extracts were had antibacterial and antioxidant properties and these properties varies according to the active ingredient’s nature or species of pathogenic bacteria and its resistance activity.